diff --git a/erpnext/accounts/doctype/sales_invoice/sales_invoice.py b/erpnext/accounts/doctype/sales_invoice/sales_invoice.py
index 0ee98114794..da95440759b 100644
--- a/erpnext/accounts/doctype/sales_invoice/sales_invoice.py
+++ b/erpnext/accounts/doctype/sales_invoice/sales_invoice.py
@@ -1634,6 +1634,21 @@ class SalesInvoice(SellingController):
 				self.company, "Sales Invoice", self.name, self.use_company_roundoff_cost_center
 			)
 
+			if self.is_opening == "Yes" and self.rounding_adjustment:
+				if not round_off_for_opening:
+					frappe.throw(
+						_(
+							"Opening Invoice has rounding adjustment of {0}.<br><br> '{1}' account is required to post these values. Please set it in Company: {2}.<br><br> Or, '{3}' can be enabled to not post any rounding adjustment."
+						).format(
+							frappe.bold(self.rounding_adjustment),
+							frappe.bold("Round Off for Opening"),
+							get_link_to_form("Company", self.company),
+							frappe.bold("Disable Rounded Total"),
+						)
+					)
+				else:
+					round_off_account = round_off_for_opening
+
 			gl_entries.append(
 				self.get_gl_dict(
 					{