diff --git a/erpnext/accounts/doctype/sales_invoice/sales_invoice.js b/erpnext/accounts/doctype/sales_invoice/sales_invoice.js
index 774d7fd5ea7..5f5a078bd4f 100644
--- a/erpnext/accounts/doctype/sales_invoice/sales_invoice.js
+++ b/erpnext/accounts/doctype/sales_invoice/sales_invoice.js
@@ -586,6 +586,8 @@ erpnext.accounts.SalesInvoiceController = class SalesInvoiceController extends (
 	set_dynamic_labels() {
 		super.set_dynamic_labels();
 		this.frm.events.hide_fields(this.frm);
+		const hide_update_stock = cint(this.frm.doc.is_debit_note) || cint(this.frm.doc.has_subcontracted);
+		this.frm.set_df_property("update_stock", "hidden", hide_update_stock);
 	}
 
 	items_on_form_rendered() {
@@ -1174,13 +1176,20 @@ frappe.ui.form.on("Sales Invoice", {
 		);
 	},
 
+	is_debit_note: function (frm) {
+		if (frm.doc.is_debit_note) {
+			frm.set_value("update_stock", 0);
+		}
+		// visibility handled by set_dynamic_labels()
+		frm.cscript.set_dynamic_labels();
+	},
+
 	refresh: function (frm) {
 		if (frm.doc.is_debit_note) {
 			frm.set_df_property("return_against", "label", __("Adjustment Against"));
 		}
 
 		frm.set_df_property("update_stock", "read_only", frm.doc.has_subcontracted);
-		frm.toggle_display("update_stock", !frm.doc.has_subcontracted);
 	},
 });
 
diff --git a/erpnext/accounts/doctype/sales_invoice/sales_invoice.py b/erpnext/accounts/doctype/sales_invoice/sales_invoice.py
index 29725897883..0e54d1553a1 100644
--- a/erpnext/accounts/doctype/sales_invoice/sales_invoice.py
+++ b/erpnext/accounts/doctype/sales_invoice/sales_invoice.py
@@ -304,6 +304,7 @@ class SalesInvoice(SellingController):
 		self.validate_uom_is_integer("uom", "qty")
 		self.check_sales_order_on_hold_or_close("sales_order")
 		self.validate_debit_to_acc()
+		self.validate_debit_note_with_update_stock()
 		self.clear_unallocated_advances("Sales Invoice Advance", "advances")
 		FixedAssetService(self).validate_fixed_asset()
 		FixedAssetService(self).set_income_account_for_fixed_assets()
@@ -960,6 +961,17 @@ class SalesInvoice(SellingController):
 		if flt(self.change_amount) and not self.account_for_change_amount:
 			msgprint(_("Please enter Account for Change Amount"), raise_exception=1)
 
+	def validate_debit_note_with_update_stock(self):
+		"""Prevent stock update when Sales Invoice is marked as Debit Note."""
+		if self.is_debit_note and cint(self.update_stock):
+			frappe.throw(
+				_(
+					"You cannot update stock for a Debit Note. A Debit Note is a financial "
+					"document that should not affect inventory. Please disable 'Update Stock'."
+				),
+				title=_("Invalid Configuration"),
+			)
+
 	def validate_dropship_item(self):
 		"""If items are drop shipped, stock cannot be updated."""
 		if not cint(self.update_stock):
diff --git a/erpnext/accounts/doctype/sales_invoice/test_sales_invoice.py b/erpnext/accounts/doctype/sales_invoice/test_sales_invoice.py
index 79283dd24bf..e168a9e9df1 100644
--- a/erpnext/accounts/doctype/sales_invoice/test_sales_invoice.py
+++ b/erpnext/accounts/doctype/sales_invoice/test_sales_invoice.py
@@ -5213,6 +5213,13 @@ class TestSalesInvoice(ERPNextTestSuite):
 
 		frappe.db.set_value("Company", "_Test Company 1", "cost_center", cost_center)
 
+	def test_debit_note_with_update_stock_validation(self):
+		"""Test that saving a Debit Note with Update Stock enabled raises ValidationError."""
+		si = create_sales_invoice(do_not_save=True)
+		si.is_debit_note = 1
+		si.update_stock = 1
+		self.assertRaises(frappe.ValidationError, si.save)
+
 
 def make_item_for_si(item_code, properties=None):
 	from erpnext.stock.doctype.item.test_item import make_item